Published April 2000
by University Press of the Pacific .
Written in English
|The Physical Object|
|Number of Pages||208|
Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications. Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. Plant Cell Culture. Plant cell, tissue, and organ culture is a set of techniques designed for the growth and multiplication of the cells and tissue using nutrient solution in aseptic and controlled environmental conditions. From: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, Related terms: Alkaloid; Anabolism. This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or.
The book is a complete reference on the art and science of plant tissue culture. Dynamic advances have been made in the last 20 years in plant tissue culture technology, leading to vast economic benefits. The book incorporates abundant information on the current areas of applied research in biotechnology such as production of new hybrids, mutants and genetically engineered plants. Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing ciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. Plant cell culture is a generic description used to describe the growth of microbe-free plant material in an aseptic (sterile) environment, such as sterilized nutrient medium in a test tube. From this early work in the 20th century plant cell culture has developed far beyond. The essential elements in plant cell or tissue culture media include, besides C, H and O, macroelements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) for satisfactory growth and morphogenesis. Culture media should contain at least mM of inorganic nitrogen for satisfactory plant cell by:
cell culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis. cell culture research, have been added to this latest edition of the handbook. The handbook is intended as a guide rather than an in-depth text book of cell culture and you are encouraged to consult relevant specialised literature to obtain more detailed information. Design and Equipment for the Cell Culture Laboratory Laboratory DesignFile Size: 2MB. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Four broad classes of growth regulators or hormones are used for culture of plant cells-auxins, cytokinins, gibberellins (Fig. ) and abscisic acid. They promote growth, differentiation and organogenesis of plant tissues in cultures. Auxins: Auxins induce cell division, cell elongation, and formation of callus in cultures.